Journal: Endocrinology

Article Title: Adiponectin Exerts Neurotrophic Effects on Dendritic Arborization, Spinogenesis, and Neurogenesis of the Dentate Gyrus of Male Mice

doi: 10.1210/en.2015-2078

Figure Lengend Snippet: Antibody Table

Article Snippet: Next day, the sections were coincubated with the following Alexa Fluor-conjugated secondary antibodies for 4 hours: antirabbit Alexa Fluor 647 goat IgG, antimouse Alexa Fluor 594 goat IgG, and antirat Alexa Fluor 488 goal IgG (1:400; Life Technologies) ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Peptide/Protein Target Antigen Sequence (if Known) Name of Antibody Manufacturer, Catalog Number, and/or Name of Individual Providing the Antibody Species Raised in; Monoclonal or Polyclonal Dilution Used Ki67 Synthetic peptide conjugated to KLH derived from within residues 1200 − 1300 of Human Ki67 Anti-Ki67 antibody Abcam, Ab15580 Rabbit; polyclonal 1:1000 Doublecortin (DCX) epitope mapping at the C-terminus of Doublecortin of human origin Doublecortin antibody (C-18) Santa Cruz Biotechnology, Inc, sc-8066 Goat; polyclonal 1:200 BrdU Anti-BrdU antibody [BU1/75 (ICR1)] Accurate Chemical, YSRTMCA2060GA Rat; monoclonal 1:400 NeuN Anti-NeuN antibody, clone A60 Millipore, MAB377 Mouse; monoclonal 1:1000 GFAP Anti-glial fibrillary acidic protein (GFAP) antibody Millipore, AB5804 Rabbit; polyclonal 1:1000 Biotinylated antirabbit IgG Biotinylated goat antirabbit IgG antibody Vector Laboratories, BA-1000 Goat 1:400 Biotinylated antigoat IgG Biotinylated horse antigoat IgG antibody Vector Laboratories, BA-9500 Horse 1:400 Alexa Fluor 488 goat antirat IgG Goat antirat IgG (H + L) secondary antibody, Alexa Fluor 488 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11006","term_id":"492389","term_text":"A11006"}} A11006 Goat 1:400 Alexa Fluor 546 goat antimouse IgG F(ab')2-goat antimouse IgG (H + L) secondary antibody, Alexa Fluor 546 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11018","term_id":"492393","term_text":"A11018"}} A11018 Goat 1:400 Alexa Fluor 647 goat antirabbit IgG F(ab')2-goat antirabbit IgG (H + L) secondary antibody, Alexa Fluor 647 conjugate Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A21246","term_id":"641368","term_text":"A21246"}} A21246 Goat 1:400 Open in a separate window Abbreviation: H+L, Heavy and light chain.

Techniques: Sequencing, Derivative Assay, Plasmid Preparation